KEY APPLICATIONS The Nitrite tube test is used to detect the presence of nitrite reducing micro-organisms in:
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The Nitrite Reducing Bacteria (NRB) tube test is designed to assess the contamination of water samples with nitrite reducing bacteria. Nitrite reducing bacteria are able to metabolise nitrite, decreasing the levels in the water system. This will dramatically reduce the effectiveness of nitrite as a corrosion inhibitor. Furthermore, metabolites of this process, ammonia and nitrogen gas, are aggressive corrosive agents. Ammonia will increase the pH of the system, increasing the corrosive potential. The test consists of a pale orange medium in a clear vial with a screw top. The medium is able to detect NRB’s in an aqueous sample and provides a semi-quantitative, easily interpreted result in the form of a colour change and bubble production, after 5 days of incubation. The NRB tube test can also be used to test for de-nitrification in effluents and sewage systems etc. |
TUBE TEST CODES
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KEY APPLICATIONS The Sulphate tube test is used to detect the presence of Sulphate reducing micro-organisms in:
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The Sulphate Reducing Bacteria (SRB) tube test is designed to assess the contamination of water samples with sulphate reducing bacteria. The test contains a straw coloured medium which reacts to the production of hydrogen sulphide to give a semi-quantitative result after 5 days. The SRB test is used specifically to indicate the presence of bacteria, which under the correct conditions, are able to produce hydrogen sulphide. Hydrogen sulphide is a colourless gas which is extremely corrosive to ferrous and no ferrous metals. This can lead to holes in water systems and leaks in tankers by dissolving the surrounding metal. Metal surfaces which are prone to attack by SRB can also be tested. A swab can be used to swab surfaces or corrosion pits and the swab is then thrust into a Sulphide test tube. The test consists of a screw capped, glass tube half filled with a selective microbiological culture medium that semi-quantitatively indicates the presence of sulphide generating bacteria by the rate and extent of the development of a black colour. Sample is added to the tube which is kept warm (incubated) for up to seven days; results are examined regularly. Results are determined by the spreading black precipitation from the inoculation point though the agar. When testing samples already high in sulphide there may be a sudden blackening and of the medium, in which case, contamination can be judged by the advancement of the blackening further into the medium. The test has been calibrated against the standard Test Method for SRB, NACE TMO-194-94, using American Petroleum Institute (API) Medium. |
TUBE TEST CODES
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PDM Comparison Chart |
WHAT IS A DIPSLIDE? The definition of a dip slide, as described in the HSE approved code of practice and guidance for the control of legionella bacteria in water systems, is a means of testing the microbial content of liquids. It consists of a plastic carrier bearing a sterile culture medium which can be dipped in the liquid to be sampled. It is then incubated to allow microbial growth. The resulting microbial colonies are estimated by reference to a chart. |
Dipslides are the most common method of measuring and monitoring microbial activity within many systems and commonly cooling systems. DTK dipslides are supplied in boxes of 10 and come complete with instructions and a colour count chart which is used to determine the concentration of aerobic activity. DIPSLIDE(S) The HSE’s ACoP L8 recommends the use of dipslides to monitor the general activity of aerobic bacteria. All dip slides should be incubated at 30oC for 48 hours to obtain a true result. Dip Slides are commonly used for the detection of slime forming bacteria in cooling & industrial water systems. DTK dipslides are dipped into the water sample and incubated for 48 hours. Any bacteria in the sample water will grow and form colonies. When compared with the reference chart supplied on the dip-slide box it is possible determine the number of bacteria in the sample. Once the dipslide has been placed in contact with the sample water or surface it should be returned and secured in its container for incubation for 48 hours normally 30oC. For continuity it is important to ensure that the incubation period and temperature is consistent each time the test is performed. For mobile users DTK offer a 12V/240V dual voltage incubator. Cooling tower water should be be tested using dipslides on a weekly basis. The consistency of timing when microbiological testing is important and the sample should not be taken if biocide has recently been applied. The clarity of the sample is not indicative of bacteriological activity and the test should be carried out regardless of sample clarity. If the treatment programme is effective, the dipslide counts should be consistently low (<104). If an unusually high result is obtained, the test should be repeated as soon as possible and confirmed. Once confirmed appropriate corrective action should be taken. Go Back to view our full dipslide range. |
KEY FEATURES |
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DTK have a range of Incubators to meet your requirements. Check out the DTK Dipslide Comparator App |
DIPSLIDE CODES
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KEY FEATURES |
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DTK have a range of Incubators to meet your requirements. Check out the DTK Dipslide Comparator App |
DIPSLIDE CODES
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KEY FEATURES |
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DTK have a range of Incubators to meet your requirements. Check out the DTK Dipslide Comparator App |
DIPSLIDE CODES
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KEY FEATURES |
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DTK have a range of Incubators to meet your requirements. Check out the DTK Dipslide Comparator App |
DIPSLIDE CODES
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KEY FEATURES |
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DTK have a range of Incubators to meet your requirements. Check out the DTK Dipslide Comparator App |
DIPSLIDE CODES
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KEY FEATURES |
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What is a Dipslide? |
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DTK have a range of incubators to meet your requirements. |
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View our Dipslide Comparator App. |
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Check out our extensive range of dipslides below. |